Figure 4
ROCK inhibition abolishes myogenic responses of both WT SMTNL1+/+ and KO SMTNL1−/− mesenteric vessels. Representative recordings of third-order mesenteric arteries, from 10-week old, male WT SMTNL1+/+ (A) and KO SMTNL1−/− (B) mice, subjected to three sequential series of pressure steps (10–120 mmHg) in normal Krebs buffer (NB), with ROCK inhibitor (GSK269962A, 3 μM) in NB, and in a Ca2+-free Krebs buffer (0 Ca2+). Cumulative data expressed as the changes in % maximum passive diameter are provided for vessels isolated from WT (C) and KO (D) animals, showing mean ± SEM (n = 3 separate animals). The effect of GSK269962A treatment as the % inhibition of myogenic tone is provided in (E). The values for myogenic constrictions were calculated as the difference between WT or KO diameter (in the absence/presence of GSK269962A) and the corresponding passive diameter for a given luminal pressure. Results were analyzed by two-way ANOVA and Sidak’s multiple comparisons test, *statistically significant differences between NB and ROCK inhibition conditions (p < 0.05). No statistically significant difference was identified for ROCK-dependent inhibition of myogenic tone between WT and KO (p > 0.05).
