Figure 2 | Scientific Reports

Figure 2

From: C/EBPβ regulates Vegf gene expression in granulosa cells undergoing luteinization during ovulation in female rats

Figure 2

C/EBPβ and HIF1α protein expressions in rat GCs undergoing luteinization. Whole cell lysate were isolated from the GCs (0 h and 12 h). Protein levels of C/EBPβ (A) and HIF1α (B) were examined by Western blotting. Protein levels of histone H3 were also assessed to ensure equal amount of proteins. Western blotting was repeated 3 times, and the representative immunoblots are shown. Quantification of bands were performed by using ImageJ and normalized with histone H3 levels. Data were shown as a ratio of those of 0 h. Each value represents the mean ± SEM of 3 independent experiments. a, P < 0.05 vs. 0 h.

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