Figure 6 | Scientific Reports

Figure 6

From: Synthetic CpG-ODN rapidly enriches immune compartments in neonatal chicks to induce protective immunity against bacterial infections

Figure 6

Flow cytometric examination of immune cells in organs. Spleen (a) and lung (b) T cell populations in day-21 embryo (72 hr post CpG-ODN or saline injection). T lymphocytes were analyzed gating on lymphocytes population based on forward and side scatter plot. CD4+ and CD8+ T-cells were quantified using PE-labelled mouse anti-chicken CD4 and FITC-labelled mouse anti-chicken CD8 monoclonal antibodies. The bar diagrams (c) spleen and (d) lung show the mean percentages of CD4+ T-cells and CD8+ T-cells 72 hr following in ovo treatment. Vertical line and horizontal bar show the standard error of mean SEM, n = 3–4. Two way ANOVA following Bonferroni post-test was done to compare means of T cell percentages of CpG-ODN received groups with saline control. Asterisks indicate significant differences between the groups, *P < 0.05, ***P < 0.001.

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