Table 1 The general utility and amplification profile of 21 SSR primer pairs based on a capillary electrophoresis (CE) detection platform.
No. | Markersa | Size range (bp) | Number of original bands | Number of detected bands | Absent alleles (bp)b | New alleles (bp)b | PIC |
|---|---|---|---|---|---|---|---|
1 | SMC119CG | 104–135 | 5 | 6 | ND | 104 | 0.92 |
2 | SMC1604SA | 105–130 | 6 | 7 | 109,124 | 105,107,110 | 0.93 |
3 | SMC1751CL | 132–160 | 5 | 7 | ND | 132,138 | 0.94 |
4 | SMC18SA | 135–150 | 5 | 6 | ND | 135 | 0.93 |
5 | SMC22DUQ | 125–165 | 7 | 8 | 125 | 142146 | 0.94 |
6 | SMC24DUQ* | 124–150 | 6 | 10 | ND | 124,133,139,150 | 0.95 |
7 | SMC278CS | 138–182 | 9 | 9 | 140,153,176 | 138,164,172 | 0.94 |
8 | SMC31CUQ* | 135–180 | 11 | 12 | 138 | 135,169 | 0.95 |
9 | SMC334BS | 143–165 | 6 | 7 | ND | 143 | 0.94 |
10 | SMC336BS* | 140–185 | 11 | 10 | 154 | 0 | 0.95 |
11 | SMC36BUQ | 100–125 | 3 | 4 | ND | 102 | 0.80 |
12 | SMC486CG | 220–245 | 5 | 5 | 227 | 235 | 0.91 |
13 | SMC569CS | 165–225 | 5 | 5 | 167,170,222 | 165,202 | 0.82 |
14 | SMC597CS* | 140–180 | 11 | 13 | ND | 150,152 | 0.95 |
15 | SMC703BS* | 200–225 | 9 | 11 | ND | 204,218 | 0.95 |
16 | SMC7CUQ | 140–170 | 6 | 7 | 158 | 143,160 | 0.90 |
17 | SMC851MS | 125–145 | 6 | 7 | ND | 138 | 0.94 |
18 | mSSCIR3* | 140–190 | 10 | 10 | 141,145 | 149,169 | 0.95 |
19 | mSSCIR43* | 200–255 | 9 | 10 | 209 | 203,229 | 0.95 |
20 | mSSCIR66 | 120–145 | 4 | 7 | ND | 125,136,142 | 0.94 |
21 | mSSCIR74 | 210–232 | 5 | 6 | ND | 214 | 0.94 |
Total | 144 | 167 | |||||
Average | 7.95 | 0.92 |
