Figure 2
From: Biochemical and Proteomic Characterization of Recombinant Human α/β Hydrolase Domain 6
Recombinant Δ29, 37 and 42 hABHD6 truncated variants characterization. (A) Activity of the hABHD6 TM truncated variants in the fluoroscent assay. (B) SDS-PAGE analysis of purification of hΔ29-ABHD6: using Talon Co2+ resin, (1) - soluble cells lysate, (2) - unbound to resin fraction, (3–5) – resin washes 1–3, (6–8) - imidazole elutions 1, 3 and 5; and using cOmplete Ni2+ resin, (9–11) - imidazole elutions 1, 3 and 5, (12) - Talon resin 200 mM EDTA wash following imidazole elutions.
