Figure 3
From: Biochemical and Proteomic Characterization of Recombinant Human α/β Hydrolase Domain 6
The hΔ29–3-ABHD6 variant characterization. (A) Primary sequence of full-length hABHD6 and the hΔ29-3-ABHD6 variant (deleted amino acid residues are marked in red). (B) Comparison of activity of full-length and hΔ29-3-ABHD6 variant using fluorogenic substrate AHMMCE. (C) Sub-cellular localization of full-length and hΔ29-3-ABHD6 variants based on activity profile. (D) Purification of hΔ29-3-ABHD6. Lanes are: (1) - total lysate, (2) - soluble fraction, (3) - insoluble fraction, (4) - unbound to resin fraction, (5–7) - resin washes 1–3 and (8–11) - elutions 1–4.
