Table 1 Enzyme Kinetics of Full Length ABHD6 and Δ29-3-hABHD6 Variant.
From: Biochemical and Proteomic Characterization of Recombinant Human α/β Hydrolase Domain 6
Enzyme Assay (Substrate) | Full-length hABHD6 ± SEM | hΔ29-3-ABHD6 ± SEM | ||
|---|---|---|---|---|
Km (μM) | Vmax (μM/hr/mg) | Km (μM) | Vmax (μM/hr/mg) | |
HPLC (2-AG) | 144 ± 10 | 24 ± 0.49 | 139 ± 4.0 | 21 ± 0.24 |
Colorimetric Gycerol (2-AG) | 200 ± 38 | 21 ± 1.92 | N/A | N/A |
Fluorescent (AHMMCE) | 26 ± 1.2 | 2.5 ± 0.07 | 20 ± 1.7 | 5.7 ± 0.04 |
