Figure 2
From: Cancer cell migration on elongate protrusions of fibroblasts in collagen matrix
Panc-1 cells actively adhere to and migrate on strings of fibroblast protrusions in 3D collagen matrix. (a) Panc-1 cells (green) without (left) or with (center and right) WI-38 cells were inoculated and incubated in collagen gel. Scale bars, 100 μm. (b) Chimeric spheroids of Panc-1 and WI-38 cells were incubated in collagen gel. Fluorescent mages after 20 h (day 1) (left panel) and 40 h (day 2) (right panel) in incubation are shown. Inset at Day 1, the original spheroid at day 0. Scale bars, 50 μm. (c,d) Magnified time-lapse images in 30-min intervals of the boxed fields in (b) after 20-h (c) or 33-h (d) incubation. Arrows trace one cancer cell migrating on a fibroblast (c) or leaving a fibroblast (d). See also Video 3. In (a) and (b), small arrows point cancer cells binding to fibroblasts.
