Table 2 Lung parameters and bronchoalveolar lavage (BAL) data in regular chow diet, HFD, HFD + high fructose, HTFD + high fructose ad libitum (O) and restricted (R) groups.
CD | Obese | Food Restricted | ||||||
|---|---|---|---|---|---|---|---|---|
HFD (O) | HFD + HFr (O) | HTFD + HFr (O) | HFD (R) | HFD + HFr (R) | HTFD + HFr (R) | |||
Number of mice (n) | 16 | 8 | 8 | 8 | 8 | 8 | 8 | |
Age (weeks) | 8 | 8 | 8 | 8 | 8 | 8 | 8 | |
Triglycerides in lung tissue (µg/mg) | 4.91 ± 0.38 | 5.82 ± 0.74 | 8.65 ± 1.17*** | 4.98 ± 0.45 | 5.8 ± 0.43 | 3.44 ± 0.29 | 5.15 ± 0.46 | |
Free fatty acids in lung tissue (umol/mg) | 0.022 ± 0.002 | 0.025 ± 0.003 | 0.026 ± 0.003 | 0.022 ± 0.001 | 0.020 ± 0.002 | 0.022 ± 0.003 | 0.03 ± 0.003 | |
Lung volume (ml) | 0.23 ± 0.02 | 0.22 ± 0.01 | 0.21 ± 0.05 | 0.23 ± 0.01 | 0.22 ± 0.01 | 0.24 ± 0.01 | 0.23 ± 0.01 | |
Interleukin-1β mRNA in epididymal fat (fold change) | 1.00 ± 0.15 | 1.01 ± 0.16 | ND | ND | ND | ND | ND | |
Interleukin-1β mRNA in inguinal fat (fold change) | 1.00 ± 0.33 | 0.44 ± 0.17 | ND | ND | ND | ND | ND | |
BAL cell count (cells/ml) | 27703.1 ± 4571.1 | 58531.3 ± 6299.4 | 61406.3 ± 13597.3 | 87268.8 ± 20707.6*** | 20714.3 ± 3965.1 | 41666.7 ± 10389.3 | 22500 ± 3273.3 | |
BAL differential (% of total) | Epithelial cells | 2.3 ± 0.5 | 1.5 ± 0.2 | 1.3 ± 0.3 | 1.5 ± 0.5 | 2.5 ± 0.6 | 0.7 ± 0.3 | 3.5 ± 1.1 |
Macrophages | 97.0 ± 0.5 | 97.9 ± 0.3 | 98.1 ± 0.4 | 97.7 ± 0.7 | 96.7 ± 0.5 | 98.9 ± 0.4 | 96.0 ± 1.3 | |
Eosinophils | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Basophils | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
Neutrophils | 0.07 ± 0.05 | 0.11 ± 0.06 | 0.08 ± 0.05 | 0.49 ± 0.33 | 0.03 ± 0.03 | 0 | 0.1 ± 0.1 | |
