Figure 1

cDNA constructs that were cloned in the pCS2(+)-V5 plasmid. (A) Diagram of the DUX4-FL protein showing the two homeodomains (HOX1, HOX2), the mid-region, and the C-terminal domains (e.g., DC1 and DC2), as well as the linker (unlabeled green box) and the V5 epitope tag (V5) added to the C-terminus. The extents of the full-length (DUX4-FL, amino acids 1–424) and short (DUX4-S, amino acids 1–159) isoforms are indicated. (B) Table of cDNA constructs that were generated, cloned into the pCS2(+)-V5 host plasmid, and used in this study. Details of the mutations introduced into the HOX1 and HOX2 homeodomains to eliminate DNA binding were described previously¹⁹.