Figure 1 | Scientific Reports

Figure 1

From: Targeting HIV-1 Protease Autoprocessing for High-throughput Drug Discovery and Drug Resistance Assessment

Figure 1

AlphaLISA detection. (A) Schematic illustration of the fusion precursors used for autoprocessing study in this report. (B) Detection principle of the full-length fusion precursor by AlphaLISA. (C) AlphaLISA detection of the indicated purified proteins spiked into the crude lysates made from the mock transfected cells at serial dilutions. (D) AlphaLISA detection of the target proteins in crude lysates. HEK 293T cells were transfected with the expression plasmids (GST-Flag or GST-p6), lysed in situ with the AlphaLISA assay buffer, and further diluted at serial dilutions prior to AlphaLISA detection. The lysate input was calculated based on cell confluency and buffer volume at the time of lysis to represent number of cells used for AlphaLISA detection.

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