Figure 3
Sections of liver, skin and lung of a mouse are imaged using a 20x objective of a confocal microscope, after perfusion with MHI148-PEI and clearing with ECi. (a) Thin section from liver in 3D (left). Zoomed-in view shows central vein indicated by an arrowhead and capillary vessel network in true color (middle). A scanned depth of 180 μm was used as indicated by the depth bar with color coding (right). (b) Thin skin section from upper back of a mouse in 3D (left). Zoomed-in view shows a vessel with some branches (middle). A thin section of depth about 70 μm was scanned as shown in the depth bar with color coding (right). (c) Thin section from lung (left). Zoomed-in view shows alveoli as indicated by an arrowhead (middle). The depth bar with color coding is on the right hand side. The colored data in 3D are the structures and the black color is used for the background in each subfigure.
