Figure 1 | Scientific Reports

Figure 1

From: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test

Figure 1

Schematic overview of the developed BacR HDA-strip assay (a) with incorporated HDA primer and strip test probe sequences (b). (a) (Step1) Asymmetric HDA amplification of the BacR marker on a heating block. (Step 2) Sequence-specific detection of HDA-derived single-stranded BacR marker copies via the nucleic acid lateral-flow strip test and visual readout of results. (b) Primer and probe binding sites of the HDA-strip and qPCR assay (GenBank accession number AF233400, bases 89 to 206). HDA primer and strip test probe regions (boxed) stretch over the qPCR primer and probe regions (marked in grey). The HDA amplicon is slightly shorter than the qPCR amplicon (115 bp versus 118 bp).

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