Table 2 Sample limit of detection experiments with the HDA-strip and qPCR method.

From: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test

Source

Faecal dilution

HDA-strip

qPCR

Detected concentration (10-8 g faeces per filter)

Filter 1

Filter 2

Filter 1

Filter 2

HDA-strip

qPCR

SLOD95%

95% CI

SLOD95%

95% CI

Cattle

10−2

10/10

10/10

10/10

10/10

4.9

2.4–10.4

2.8

1.3–5.9

10−3

10/10

9/10

10/10

10/10

10−4

4/10

8/10

5/10

10/10

10−5

0/10

2/10

1/10

2/10

10−6

0/10

0/10

0/10

0/10

Sheep

10−2

10/10

10/10

10/10

10/10

2.5

1.2–5.2

2.5

1.2–5.2

10−3

10/10

10/10

10/10

10/10

10−4

4/10

10/10

6/10

10/10

10−5

1/10

4/10

1/10

2/10

10−6

0/10

0/10

0/10

0/10

Fallow Deer

10−2

10/10

10/10

10/10

10/10

11.3

4.9–25.9

12.6

5.5–29.1

10−3

9/10

9/10

9/10

10/10

10−4

0/10

8/10

1/10

7/10

10−5

1/10

2/10

0/10

1/10

10−6

0/10

0/10

0/10

0/10

  1. The results are given as the number of positive replicates from the analyses of 10 replicates per faecal dilution step. The total number of replicates from both filtrations (i.e., n = 20 replicates per faecal dilution) served as input for statistical analysis. Detection limits were calculated as the minimum amount of faeces detectable in a filtration volume at a 95% detection probability (SLOD95%) with a 95% confidence interval (CI).
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