Table 1 The experimental molecular masses, affinities and melting temperatures of the conjugates.

From: Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

 

Mw (Da)

KD (pM, mean ± SD)* 1500 RU

KD (pM, mean ± SD)** 2500 RU

Tm (°C)

Z08698-DOTA

7320.6 (7320.2)

12 ± 2.6

13 ± 2.9

64.5

Z08698-DOTAGA

7392.7 (7392.3)

15 ± 0.4

16 ± 0.8

64.0

Z08698-NOTA

7219.6 (7219.1)

40 ± 1.5

45 ± 6.7

63.8

Z08698-NODAGA

7291.5 (7291.2)

11 ± 0.6

13 ± 0.05

64.3

  1. The theoretical molecular mass is in parenthesis.
  2. *His-HER3 immobilized surface with 1500 RU (resonance units).
  3. **mFc-HER3 immobilized surface with 2500 RU.
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