Table 3 In vitro binding kinetics.

From: Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

 

111In-Z08698-NOTA

111In-Z08698-NODAGA

111In-Z08698-DOTA

111In-Z08698-DOTAGA

ka (1/M*s)

10 × 104 ± 2 × 104

1.7 × 105 ± 0.8 × 105

1.5 × 105 ± 0.4 × 105

1.3 × 105 ± 0.8 × 105

kd (1/s)

1.4 × 10−5 ± 0.7 × 10−5

2 × 10−5 ± 3 × 10−5

1.3 × 10−5 ± 0.7 × 10−5

0.23 × 10−5 ± 0.08 × 10−5

KD (pM)

160 ± 50

21 ± 7

80 ± 50

8 ± 6

  1. Equilibrium dissociation constant (KD), association rate (ka) and dissociation rate (kd) of 111In-Z08698-X. ka, kd and KD were measured in real time on BxPC-3 cells at room temperature.
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