Figure 4
From: Discovery and evaluation of inhibitor of LARP6 as specific antifibrotic compound
Suppression of type I collagen production by hepatic stellate cells (HSCs). (A) Inhibitory effect of C9 in HSCs during differentiation from quiescent into activated phenotype. Primary rat HSCs at day 3 in culture were treated with indicated concentrations of C9 and cellular medium was analyzed for accumulation of collagen α1(I) polypeptide (COL1A1) by western blot at day 5. Loading control, fibronectin (FIB). (B) Inhibitory effect in activated HSCs. HSCs at day 5 in culture were treated with indicated concentrations of C9 until day 7 and cellular medium was analyzed for collagen α1(I) (COL1A1) and α2(I) polypeptide (COL1A2) by western blot. FIB, fibronectin as loading control. (C) Effect on expression of α-SMA in two independent experiments. Primary rat HSCs at day 3 in culture were treated with indicated concentrations of C9 and cellular extract was analyzed at day 5 for expression of α-SMA, COL1A1 and actin (ACT) as loading control.
