Figure 5
From: Discovery and evaluation of inhibitor of LARP6 as specific antifibrotic compound
Reversible effect of C9 on type I collagen production. (A) Suppression of type I collagen production by HLFs. HLFs were treated with indicated concentrations of C9 overnight and cellular medium was analyzed for collagen α1(I) (COL1A1) and α2(I) polypeptide (COL1A2) by western blot. FIB, fibronectin as loading control. (B) Removal of C9 reverses the inhibitory effect. Upper panel: HLFs were treated with indicated concentrations of C9 overnight and cellular medium analyzed for collagen α2(I) polypeptide (COL1A2). Lower panel: C9 was washed off from the cells, the cells were incubated for additional 24 h and the medium was analyzed for collagen α2(I) polypeptide (COL1A2). FIB, fibronectin as loading control.
