Figure 7
From: Discovery and evaluation of inhibitor of LARP6 as specific antifibrotic compound
Prophylaxis of hepatic fibrosis by C9. (A) Representative images of Sirius red staining of liver sections (additional images are shown in the supplemental material). Upper left panel: normal liver. Upper right panel: fibrosis after 4 weeks in untreated animal. Lower left panel: fibrosis after 4 weeks in C9 treated animal. Lower right panel: liver of an animal receiving only C9 for 4 weeks. The scale bar (1 mm) is shown in the first image, all other images were taken under the same magnification. (B) Quantification of fibrosis. Left panel: morphometric quantification of fibrosis area from Sirius red stained histological slides. Significant reduction of fibrosis between nontreated and treated animals is indicated (p < 0.0001, unpaired t test). (C) Determination of hydroxy-proline content of the livers. Hydroxyproline was measured by colorimetric assay in duplicate and plotted (p = 0.0323, unpaired t test). Number of animals in each cohort is specified. Error bars: +−SEM. (D) Measurement of type I collagen and α-SMA by western blot. Liver samples were homogenized and total proteins were analyzed by western blot. Procollagen is indicated by bracket. α-smooth muscle actin (αSMA) was analyzed in the same samples and tubulin (TUB) is shown as loading control. (E) Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured in plasma of the indicated cohorts of animals. Box plots showing median, first quartile, third quartile and minimal and maximal values are shown.
