Figure 1

Experimental strategy and the basic information of LC-MS/MS data. (a) Experimental strategy for lysine succinylation identification was showed. Total proteins from P. oryzae were isolated and trypsin digested. All succinylated peptides were enriched by an antibody and analyzed by LC-MS/MS method. (b) Western-blot using a succinyl-lysine specific recognized antibody was used to check the extent of lysine succinylation on the total proteins of P. oryzae. (c) The peptides score of LC-MS/MS data. (d) Length distribution of succinylated peptides based on their length. (e) The number of proteins with different number of succinylated sites.