Figure 7

Binding of PPARγ to PPARγ response element-like (PPRE) sequences located in cathepsin B and L promoters. Electrophoretic mobility shift assays were performed using the end-labeled oligonucleotides representing the wild-type (wt) consensus PPRE, the human putative CatB PPRE-like sequences, the human putative CatL PPRE-like sequences (lanes 1, 2, 6 & 7) and mutated (mut) versions of these three sequences (lane 5) in the presence of human recombinant PPARγ and RXRα. Molar excess 200-fold (lanes 3 & 4) of unlabeled oligonucleotides was used for competition analysis. Supershift assays were performed using an anti-human PPARγ (lanes 8 & 9). Black arrows indicate specific gel shifts and white arrows indicate the supershifted bands (a representative sample is shown, n = 3). Full-length blots are presented in Supplementary Fig. 5.