Figure 1
From: Arrestin-1 engineering facilitates complex stabilization with native rhodopsin
Half-maximal inhibitory concentration (IC50) values of sodium chloride for formation of complexes composed of mutant arrestin-1 and both phosphorylated and light-activated rhodopsin. Double mutants (blue circles) are sorted by decreasing IC50 value (from left to right) and are composed of F375A + X, where the single mutant X is shown underneath and named on the x-axis. Triple mutants are composed of either F375A + A307G + X or F375A + T304A + X, while quadruple mutants are combinations of F375A + T304A + E341A + X or F375A + T304A + F380A + X. The size of the shape encodes the functional expression level of the respective mutant and is scaled in relation to the WT arrestin-1 functional expression level (legend on the top right). IC50 values, functional expression level of single13,14 and combined mutants are listed in SI Table 1.
