Figure 5

FM1–43 dye uptake in P1 WT (a,c) and KO mice (b,d). Panel a shows dye uptake in the middle turn of WT cochlear OHCs. There was also uptake in the IHCs in WT; however, it is invisible in this picture because IHCs were in different focus plane. No uptake in KO cochlear hair cells (b). Panels c and d are DIC images of same regions of HC epithelium as shown in a and b, respectively. Dotted lines in b & d delineate the medial (lower) and later (upper) boundaries of the organ of Corti. The scale bars = 20 μm.