Figure 6

icLNPs and cLNPs are stable in resting platelets but the delivered mRNA is not translated. (a) Quantifying the amount of mRNA using qPCR after excess LNPs were removed (n = 12). (b) Quantifying the number of Cy5-positive platelets 4 h after excess LNPs were removed and platelets were resuspended in Tyrode’s buffer at pH 6.5 or pH 7.4 or in plasma, using flow cytometry (n = 5). (c) Assessing the amount of RNA that remains inside platelets during storage for 2 h under different conditions by quantifying biotin-labelled RNA in confocal immunofluorescence images of platelets (n = 5). (d) HEK293 cells (n = 8) and (e) platelets (n = 5) were transfected with LNPs containing GFP mRNA and after 24 h protein expression was quantified by measuring green fluorescence. N.S. means not significant. Error bars represent SEMs. *P < 0.05, **P < 0.01, ***P < 0.001.