Figure 3 | Scientific Reports

Figure 3

From: Prominent action of butyrate over β-hydroxybutyrate as histone deacetylase inhibitor, transcriptional modulator and anti-inflammatory molecule

Figure 3

Effects of NaBut or NaR/S-β-hydroxybutyrate supplementation throughout L6 cells differentiation. L6 myoblasts were differentiated for 7 days into myotubes in the presence of increasing concentrations of NaBut or NaR/S-βOHB. Acid-extracted histones were immunoblotted with antibodies anti-acetyl lysine, anti-H3K9/14Ac, anti H3K9Ac and anti H3K9Me2. Loading was assessed by anti total histone H3 immunoblotting and coomassie staining of extracted histones. Signal quantification, relative to total H3 immunoblot signals, from representative western blots is shown on the right.

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