Figure 8

NaBut, but not NaR-βOHB (R-βOHB in graphs) affect transcription of multiple genes related to mitochondrial function. (A) Quantification of mRNA levels by RT qPCR for PGC1α CPT1b and SIRT1 in L6 myotubes treated for 24 h with 5 mM NaBut (grey bars) or 20 mM NaR-βOHB (black bars). (B) Quantification of mRNA levels of mitochondrial Sirtuins 3,4 and 5 in L6 myotubes treated for 24 h with 5 mM NaBut (grey bars) or 20 mM NaR-βOHB (black bars). (A,B) Data are presented and analyzed as in Fig. 6. (C) Mitochondrial to nuclear genome ratio as measured by amplification of two mitochondrial gene loci (Cox2 and Cox1) and a nuclear locus (on the Ppia gene) in a control or 5 mM NaBut conditions. Data are presented and analyzed as in Fig. 6.