Table 2 sequences of primers used in this study for mRNA quantifications in L6 (upper table) and HMEC-1 cells (lower table).
Gene | Sequence | Amplicon size bp | Annealing temperature |
|---|---|---|---|
Rat L6 cells. | |||
Hprt1 | F: TTGCTGACCTGCTGGATTAC R: AGTTGAGAGATCATCTCCAC | 151 pb | 55 °C |
Glut4 | F: GGGTTTCCAGTATGTTGCGG R: CTGGGTTTCACCTCCTGCTC | 183 pb | 60 °C |
Hk2 | F: CTTCTCGTTCCCCTGCCACC R: CCATGTAGCAGGCGTTGCTG | 202 pb | 62 °C |
Irs1 | F: ACCAGAGGACCGTCAATAGC R: AAGACGTGAGGTCCTGGTTG | 138 pb | 60 °C |
GS | F: TCAGACCCCATCTTGACCAC R: TCCATAAAGCAGCCAAAGCC | 260 pb | 60 °C |
Gsk3β | F: TTCAGGTGGAGTTGGAAGCTGATGC R: AACACCACTGGAAGCTTGTGC | 278 pb | 62 °C |
Hmgcs1 | F: CTGGACCGCTGCTATTCTG R: TCAGGAACATCCGAGCTAGAG | 157 | 60 °C |
Eif2 | F: ACCAAGGATGAGCAGCTGGA R: ATAGATGGGTCTGAGACTGC | 122 | 58 °C |
Catalase | F: CATCGGCACATGAATGGCTA R: ACCTTGGTCAGGTCAAATGG | 281 | 60 °C |
Foxo3a | F: GAGAGCAGATTTGGCAAAGG R: CCTCATCTCCACACAGAACG | 182 | 58 °C |
SOD2 | F: TCATGCAGCTGCACCACAGC R: CCATTGAACTTCAGTGCAGG | 138 | 60 °C |
SOD1 | F: TGAAGAGAGGCATGTTGGAG R: CCACCTTTGCCCAAGTCATC | 164 | 58 °C |
Pgc1α | F: TCCTCTGACCCCAGAGTCAC R: CTTGGTTGGCTTTATGAGGAGG | 143 | 60 °C |
CPT1b | F: AGGCAGTAGCTTTCCAGTTCAC R: CACACCCCTAAGGATACCATTC | 146 | 60 °C |
Sirt1 | F: TGAGAAAATGCTGGCCTAATA R: GATAAGACGTCATCTTCAGAG | 144 | 60 °C |
Sirt3 | F: CCGACATTGTGTTCTTTGG R: TCAAGCTGGCAAAAGGCTC | 128 | 60 °C |
Sirt4 | F: TGACAGAGCTCCACGGATGC R: TTGGGACCTGAAAGCTCCGGAC | 188 | 60 °C |
Sirt5 | F: CTGGCACCAAGAACCTTCTG R: TGGGATGCTGGCATCTTGAG | 217 | 60 °C |
COX1 | F: GGTTCTTTTTTTCCGGAGTA R: ACTATACTACTACTAACAGACCG | 181 bp | 58 °C |
Ppia | F: CAGTCTTGGCAGTGCAGAT R: ACACGCCATAATGGCACTGG | 156 bp | 55 °C |
Human HMEC-1 cells | |||
Ccl2 | F: TCTCAAACTGAAGCTCGCACT R: TGGGGCATTGATTGCATCTGG | 126 bp | 59 °C |
IL-6 | F: CTCATTCTGCCCTCGAGCC R: GACCGAAGGCGCTTGTGGA | 97 bp | 60 °C |
IL-8 | F: ACCGGAAGGAACCATCTCAC R: GGCAAAACTGCACCTTCACAC | 108 bp | 59 °C |
GM-CSF | F: ACTTCCTGTGCAACCCAGATT R: CTCATCTGGCCGGTCTCAC | 120 bp | 59 °C |
IL-4 | F: ATCTTTGCTGCCTCCAAGAACAC R: TGCTTGTGCCTGTGGAACTGC | 140 bp | 61 °C |
IL-1β | F: TCGCCAGTGAAATGATGGCT R: AGATTCGTAGCTGGATGCCG | 138 bp | 60 °C |
