Figure 1
From: Nanoparticle administration method in cell culture alters particle-cell interaction
AuNP colloidal stability is independent of administration method. (a) Schematic showing the different administration method concepts. Particles can be administered to cells as a concentrated dose (left), a pre-mixed solution (center), or as a concentrated dose which is mixed via aspiration (right). (b) Transmission electron microscopy of citrate AuNP with shows a mean core diameter of 116 ± 12 nm (n = 289). Scale bar represents 200 nm. (c) UV-Vis spectra of PVP-coated AuNPs over 24 hr in cell culture media supplemented with 10% fetal bovine serum (FBS) following concentrated administration. (d) UV-Vis spectra of PVP-coated AuNPs administered via the pre-mixed method in cell culture media supplemented with 10% FBS. (e) Depolarized dynamic light scattering of PVP-coated AuNPs measured over 24 hr in complete cell culture media.
