Figure 2
Antibiotics-induced dysbiosis in wild-type and TashTTg/Tg mice at P28–30. (A) Flow cytometry-based quantitative analysis of SYTO 13-labeled bacteria in stool homogenates prepared from antibiotics-treated (Abx) mice in comparison to untreated (Ctl) mice at P14, P21 and P28. At each stage, antibiotics-induced reduction of the average concentration of bacteria per µl of stool homogenate (red numbers on the left of each panel) was determined and expressed as percentage difference (red numbers at the bottom of lower panels) of the average bacterial concentration in untreated mice. A blank solution without bacteria (negative control) was used for gating purposes. (B) Profile of bacteria extracted from stool pellets of P28–30 WT and TashTTg/Tg males exposed or not to antibiotics in their drinking water, which was sweetened with sucrose. The pie charts display the relative average abundance of 16S rRNA gene sequences at the phylum and order levels. Taxonomic groups are color-coded and indicated at the bottom of each panel. “Others” and “Unclassified” stand for the sum of low-abundance (<1.5%) taxa or yet unknown taxa, respectively. (*P < 0.05, **P < 0.01; Student’s t-test).
