Figure 5
Impact of antibiotics-induced dysbiosis on neuronal density and proportion of nitrergic neurons in the myenteric plexus of wild-type and TashTTg/Tg male mice at P30–36. (A) Neuronal density was evaluated via immunofluorescence labeling of the pan-neuronal marker HuC/D and is expressed as the number of neurons per mm2 of longitudinal surface area. (B) The proportion of nitrergic (nNOS+) neurons was evaluated by double-immunofluorescence labeling and is expressed as the percentage of nitrergic neurons among the total number of neurons. (*P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA with Tukey’s post hoc test).
