Figure 8
Impact of inhibition of NO signaling on fecal retention and life expectancy of TashTTg/Tg male mice exposed or not to antibiotics. (A) Picture of all antibiotics-treated TashTTg/Tg colons used for the quantitative analysis in B, along with their respective fecal retention score (numbers at the bottom). (B) Quantitative analyses of fecal retention (using the arbitrary units described in Fig. 3B,C) in P33 TashTTg/Tg male mice exposed to antibiotics and subjected to daily rectal enemas with L-NAME or vehicle (H2O) for 7 days starting at P26. (C) Kaplan-Meier curve showing the survival rate of megacolon-suffering P20–29 TashTTg/Tg male mice not exposed to antibiotics but subjected to daily rectal enemas with L-NAME or vehicle (H2O) for a maximum of 7 days (delineated by the grey box) starting on the day where megacolon symptoms were detected. Animal survival was then evaluated over another 3-week period, for a maximum total of 28 days post-diagnosis. (****P < 0.0001; Chi-square test).
