Figure 2 | Scientific Reports

Figure 2

From: Influence of accuracy, repeatability and detection probability in the reliability of species-specific eDNA based approaches

Figure 2

Assessment of standard curves used for quantifying M. margaritifera DNA as well as for determining the limit of detection (LOD) and limit of quantification (LOQ) with qPCR targeting the COI (A) and the 16S region (B). Standard curves were obtained with the same 1:10 dilution series from a starting concentration of 7.8 ng with 10 replicates per concentration. The threshold cycles are representing the minimum number of qPCR amplification cycles leading to positive detection. (C) The relationship between the detection probability and DNA concentration for the COI and 16s assays. (D) The coefficient of variation of eDNA measurements and its relation to DNA concentration in the standard curve. The black line represents the regression equation for the relationship. Data was pooled from both assays as results did not diverge significantly between methods.

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