Table 2 Primers and probes for the detection of environmental DNA traces released by the Freshwater Pearl mussel Margaritifera margaritifera.

From: Influence of accuracy, repeatability and detection probability in the reliability of species-specific eDNA based approaches

Margaritifera margaritifera

Target

Primers

Sequence (5′-3′)

Source

COI

Forward

TTGTTGATTCGTGCTGAGTTAGG

28

COI

Reverse

GCATGAGCCGTAACAATAACATTG

28

COI

Probe

6-FAM- CCTGGTTCTTTGCTGGGT-BHQ-1

28

16S

Forward

CAACCCTGGAACCGCTAAAG

27

16S

Reverse

GGCTGCGCTCATGTGAATTA

27

16S

Probe

6-FAM- TCCAGTTAATCATAGAACTTCATCAAA-BHQ-1

This study

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