Figure 1 | Scientific Reports

Figure 1

From: Comparison between dopaminergic and non-dopaminergic neurons in the VTA following chronic nicotine exposure during pregnancy

Figure 1

Quality assessment of DA and non-DA FACS and microarray analysis. (a) Typical FACS analysis results for sorting DA and non-DA neuron populations from VTA brain punches. DP shows the double-positive events indicating DA neurons by positive expression of TH and NeuN, while AF488 indicates the events positive only for the NeuN neuronal marker indicating the non-DA neuron population. The AF488 threshold was set to increase the specificity of non-DA neuron collection. (b) Results from principal component analysis showing clustering of the DA versus non-DA neurons as well as grouping based on saline versus nicotine treatment. Heatmaps of the microarrays showing top differentially expressed (c) miRNAs and (d) mRNAs for DA and non-DA following perinatal nicotine or saline treatment. The mRNA microarray maps also indicate a clear grouping based on neuron type.

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