Figure 2

rh-BDNF influences fibrin density and polymerization, and in vitro clot dimension in healthy subjects’ plasma. Recombinant BDNF (rh-BDNF; 60, 120 pg/ml) or BSA (1 mg/ml: control) was added to plasma pools from healthy subjects before induction of coagulation with thrombin, then fibrin density and polymerization, and viscoelastic property of clot were analyzed. (ai) Visualization images (20X magnification) with Alexa Fluor 488–labeled method and (aii) quantization of fibrin fibers using Image J software. (b) Maximum Clot firmness (MCF) assessed by thromboelastographic analyses. All samples were performed in triplicate. (c) Representative kinetic and (d) maximum turbidity detected at A350 nm at 37 °C and monitored every 23 sec by spectrophotometric method. Data are expressed as mean ± SEM; horizontal bars indicate variation of BDNF levels measured in plasma pools analyzed; n = 5 different pools. **p < 0.01.