Figure 4
Inhibition of stress responses and melanin synthesis mediated by RgsD. (A) Conidial viability of WT, ΔrgsD, and C′ strains in presence of UV irradiation and H2O2 at indicated conditions. Note that the viability of the ΔrgsD conidia was significantly increased compared to WT and C′ strain’s conidia. (B) Expression of melanin biosynthetic genes arp1 and arp 2 mRNA analyzed by qRT-PCR. Student’s t-test: **p < 0.01. (C) Melanin content in three strains’ conidia. Statistical significance was determined by a Student’s t-test: **p < 0.01. (D) TEM photographs of WT, ΔrgsD, and C′ conidia. Note that the ΔrgsD conidia exhibited higher expression of melanin synthesis-related genes’ mRNA, melanin content, and the melanized outmost cell wall than WT and C′ strain.
