Table 1 Exemplified protocols for the isolation of ECs from mouse lung and aorta.
From: A simple protocol for isolating mouse lung endothelial cells
Type of EC (tissue) | Age | Method of isolation | Sorting method | Reference |
|---|---|---|---|---|
MLECs (lung) | 3–4 month old | Collagenase A digestion of minced tissue (37 °C, 1 h) | Double magnetic bead sort (ICAM2) | |
Neonatal mice | Collagenase I/dispase digestion of minced tissue (37 °C, 45 min) | Double magnetic bead sort (CD31/ICAM2) | ||
Neonatal mice | collagenase A digestion of minced tissue (37 °C, 30 min) | FACS or magnetic beads (ICAM2) | ||
NA | Collagenase I digestion of minced tissue (37 °C, 45 min) | Double magnetic bead sort (CD31 for both sorting) | ||
7–8-week-old | Enzyme Mix plus MACS™ dissociator (37 °C, 30 min) | Magnetic cell sorting using CD146 Microbeads (Miltenyi Biotec) | ||
MAECs (aorta) | Adult | Lumen digestion (Collagenase II) (37 °C, 45 min) | NA | |
NA | Matrigel + aortic ring explant | NA | ||
4-week-old | Type I Collagen gel + aortic ring explant | NA |
