Figure 3 | Scientific Reports

Figure 3

From: Differences in the influenza-specific CD4 T cell immunodominance hierarchy and functional potential between children and young adults

Figure 3

Overall cytokine production by influenza-specific CD4 T-cells is biased away from IFNγ in pediatric subjects. Following PBMC stimulation and staining, the frequency of activated IFNγ, IL-2 and TNFα producing cells per million CD4 T-cells was determined for each subject and stimulation condition. Cells producing each cytokine were then summed across stimulation conditions to quantify the total frequency of influenza-specific CD4 T cells for each cytokine examined. Data are presented as boxplots, with each point representing a given subject. In (B) these data were normalized by dividing by the frequency of IFNγ+ producing cells to better illustrate the differences in CD4 T cell cytokine production between young adults and children. Statistical analysis was performed by two-group comparison testing with the Wilcoxon rank-sum test after data preprocessing. P-values were adjusted for multiple comparisons using the Benjamini-Hochberg procedure.

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