Figure 5 | Scientific Reports

Figure 5

From: Differences in the influenza-specific CD4 T cell immunodominance hierarchy and functional potential between children and young adults

Figure 5

Cytokine production by CD4 T cells isolated from pediatric subjects is characterized by overall less IFNγ production, with the differences in cytokine elaboration between children and adults largely consistent across protein specificities. Cell were stimulated and stained as previously described, with gating as shown in Fig. 1. Combination gates were then created using FlowJo v10 software (TreeStar) to highlight single, double, and triple cytokine producing cells. Following data preprocessing, arced pie graphs were constructed using SPICE software (NIH), with the arcs demonstrating all cells producing a given cytokine (IFNγ: green, IL-2: blue, TNFα: purple) and pie slices depicting the percentage of cells with a given cytokine expression pattern as indicated in the Figure. Data from pediatric subjects are shown in Panel A, while young adult data are shown in Panel B.

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