Figure 5
Plasma-treated saline reorganized actin-cytoskeleton. (a) Image quantification total phalloidin (staining actin) fluorescence; (b) representative confocal images of fixed CT26 cells stained against actin and DNA showing distinct pericellular patterns (scale bar = 10 µm); (c) quantification of mean cell extrusion length and (d) mean fluorescence intensity (MFI) of extrusions; all data are 4 h after exposure to saline solutions with at least 2000 individual cells analyzed per condition. Data are presented as mean and SEM of one representative of three independent experiments; statistical analysis was carried out with ANOVA; P0 = control PBS, P20 and P60 = plasma-treated PBS, H100 = concentration-matched hydrogen peroxide to P60.
