Figure 7
Plasma-treated saline increased translocation and expression of immunogenic cell death (ICD) surface markers in CT26 cells. Representative flow cytometry histograms of CT26 cells stained against the ICD markers calreticulin (CRT; a), HSP70 (c) and HMGB1 (e) to control or plasma-treated PBS and their respective quantification (b,d,f) showed an increased expression of surface markers, especially after 24 h; (g) representative DPC and HMGB1 (AF647) images (scale bar = 10 µm); (f) ratio of mean fluorescence intensity of HMGB1 in nuclear and cytosolic area 24 h after treatment; imaging data are from of nine fields of view in 4 technical replicates per condition. Data (b,d,f) are mean and SD of three independent experiments; statistical analysis was carried out with ANOVA; P0 = control PBS, P20 and P60 = plasma-treated PBS, H100 = concentration-matched hydrogen peroxide to P60.
