Figure 8

Oxidized saline decreased tumor burden in vivo and increased macrophage count and T cell activation. (a) Representative image of intraperitoneal burden of CT26 colon cancer cells in Balb/C mice; (b) tumor weight in animals receiving saline (untreated) or plasma-treated saline; (c) gating of intratumoral macrophages (F4/80⁺ cells of CD45⁺ leukocytes) of digested tumors assessed via flow cytometry; (d) quantification of intratumoral macrophages; (e) pooled (one sample from two mice of the same group) splenic CD4⁺ T cell activation (CD25 mean fluorescent intensity × % CD25⁺) of animals receiving saline (untreated) or plasma-treated saline after 24 h of incubation with heat-killed CT26 tumor cells ex vivo (p < 0.018, ratio-paired t test). Data (b,d) are mean of 8–9 animals, results were statistically compared with t test.