Figure 4
A Shift of NG-to-HG or HG-to-NG activates the signaling kinases of MAPKs and Akt in cultured BV-2 microglia. (a) The media of BV-2 cells assigned as NG- and HG-cultured cells was simply renewed using fresh NG and HG media or replaced by HG and NG media, respectively. Next, cultures were incubated for 10, 20, 40, or 60 min. After harvest, western blotting was performed using specific antibodies for pERK, ERK, pJNK, JNK, pp38, p38, pAkt, and Akt (for uncropped images of the blots please see Supplementary Information). GAPDH served as a protein loading control. The representative blots from one of four independent experiments are presented. (b–e) The expression levels of pERK, pJNK, pp38, and pAkt were quantified. The histograms show the relative levels obtained using densitometry to quantify western blot band intensity, followed by normalization to the corresponding total protein level. The quantitative values were expressed relative to the constant NG group (assigned a value of 1). Data are represented as mean ± SEM from four independent experiments. *p < 0.05 versus constant NG group.
