Figure 2
From: Connexin43 mutations linked to skin disease have augmented hemichannel activity
Cx43 mutations do not alter gap junction voltage gating. Oocyte pairs were subjected to hyperpolarizing and depolarizing transjunctional potentials (Vj) while recording junctional currents (Ij). (a) Wild-type Cx43 gap junction channels had Ijs that decreased symmetrically at higher values of Vj. Ijs between cell pairs expressing Cx43-G8V (b), Cx43-A44V (c), or Cx43-E227D (d) behaved in a similar fashion. (e) Steady-state voltage gating of wild-type Cx43 (filled squares) showed an approximately symmetric decline in steady state conductance at increasing values of Vj. Data from cell pairs expressing Cx43-G8V (open squares), Cx43-A44V (open circles), or Cx43-E227D (open triangles) were similar to wild-type Cx43.
