Figure 5
From: Connexin43 mutations linked to skin disease have augmented hemichannel activity
Representative examples of patch clamp recordings from cell-attached patches containing single Cx43-G8V (a), Cx43-A44V (b), and Cx43-E227D (c) hemichannels in symmetric 140 mM KCl solutions. Single hemichannel currents were recorded in response to 8-s voltage ramps between −70 and +70 mV. Current-voltage relations for Cx43-A44V and Cx43-E227D were linear, whereas those of Cx43-G8V showed slight inward rectification. All three mutations show closing transitions to subconductance states. Occasional transitions to the fully closed state are also seen (b). (d) Cell-attached patches from wild-type Cx43 injected oocytes failed to show single channel activity. (e) Mean slope conductances measured at Vm = 0 are similar for the three mutant hemichannels. Values represent the mean ± SEM from 5 to 7 recordings.
