Figure 3
Gγ subunit specificity to α2a adrenergic receptors. Quantification of Gγ subunit interactions with α2aARs in both adrenergic and non-adrenergic neurons (N = 4 unless otherwise noted on the graph). Gγ subunits detected (fmol) from quantitative measurements were normalized by the amount of protein (mg), calculated using the volume of precleared lysate used and the protein concentration of precleared lysate from BCA assay, used in co-IPs. Several controls were run: unstimulated WT (WT no epi), HA-α2aAR (HA-α2aAR no epi), and stimulated WT (WT + epi) samples. These are all controls for the key sample, the Gβ and γ isoforms interacting with HA-α2aAR. Gγ2, Gγ3, Gγ4, and Gγ12 specifically interact with HA-α2aARs present in all synaptic terminals. Data were presented as mean ± SEM and compared by one-way ANOVA, *P < 0.05 and **P < 0.01. Post hoc analysis was performed with Tukey’s multiple comparison test.
