Figure 5
Gγ subunit specificity to auto-α2a adrenergic receptors. Quantification of Gγ subunits interacting with auto-α2aARs on adrenergic neurons (N = 5 unless otherwise noted on the graph). The data were analyzed identical to the study of α2aARs in both adrenergic and non-adrenergic neurons. Unstimulated α2aARs KO (KO no epi), FLAG-α2aAR (FLAG-α2aAR no epi), and stimulated KO (KO + epi) samples are controls. The difference between these epi-stimulated α2aARs KO and FLAG-α2aAR represents the interaction of Gγ isoforms upon auto-α2aARs activation. Gγ2, Gγ3, and Gγ4 specifically interact with auto-α2aARs. Data were presented as mean ± SEM and compared by one-way ANOVA, *P < 0.05 and **P < 0.01. Post hoc analysis was performed with Tukey’s multiple comparison test.
