Figure 1 | Scientific Reports

Figure 1

From: Enhanced Macrophage Pannexin 1 Expression and Hemichannel Activation Exacerbates Lethal Experimental Sepsis

Figure 1

An exogenous bacterial endotoxin (LPS) and an endogenous inflammatory cytokine (SAA) up-regulated Panx1 expression in macrophage (Mϕ) and monocyte (MC) cultures. Murine macrophage-like RAW264.7 cells (Panel a), differentiated human macrophages (Panel b), human peripheral blood mononuclear cells (MC, Panel c), or primary murine peritoneal macrophages (Panel d) were stimulated with crude LPS or SAA at indicated concentrations for indicated time periods, and the cellular Panx1 levels were measured by Western blotting (Panel a,b,c) or immunocytochemistry (Panel d), respectively. A house-keeping gene product β-actin was used as a loading control to estimate the relative Panx1/β-actin (P/A) ratio in macrophage or monocyte cultures.

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