Figure 2
Crude LPS and purified SAA induced Panx1 release in macrophage and monocyte cultures. (a,b) Extracellular release of Panx1 by murine macrophages. Murine macrophage-like RAW264.7 cells were stimulated with LPS or SAA at indicated concentrations for 16 h. The macrophage-conditioned culture medium was subjected to differential centrifugations, and each fraction was assayed for Panx1 by Western blotting analysis. Note Panx1 was found in the LPS- or SAA-stimulated macrophage-conditioned medium (“Cell Medium”), and the supernatant of the 20,000 × g centrifugation. (c,d) Extracellular release of Panx1 by primary human monocytes. Human PBMCs were stimulated with crude LPS at indicated concentrations for different time periods, and extracellular Panx1 levels were determined by Western blotting analysis. The relative optical intensity of the 48-kDa (Panx1) and 12-kDa (Panx1Δ) band was measured, and expressed as an arbitrary unit (AU).
