Figure 5
From: Metformin prevents cell tumorigenesis through autophagy-related cell death
Evaluation of molecular changes in 3T3-619C3 and 3T3-SCRD3 cells. Western blotting analysis of ATG7, phospho-ERK1/2, caspase-3 cleavage, GRP78/Bip, CHOP (A) and p62 (B) in cells cultured overnight with H2O2 (10 µM) and treated with metformin (10 mM) for 24 (A,B) and 48 (B) hours. (C) Western blotting analysis of caspase-3 cleavage in 3T3-SCRD3 cells treated with cisplatin and/or zVAD-fmk for 48 hours. Total ERK1/2 (A) and actin (A,B,C) were used as loading control. N = 3; Cropped blots are shown; Uncropped blots are presented in Supplementary Fig. S3. (D) Gene expression analysis of shATG7 and scrambled transfected NIH/3T3 cells cultured for 24 hours with H2O2 (10 µM) and treated with metformin (10 mM) for 4 hours. Data are means ± SD. N = 3; *P < 0.05, **P < 0.01, ***P < 0.001.
